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Lugar para Tesina - IBR-CONICET

Se ofrece un lugar para realizar la tesina de grado en el laboratorio de Parasitología Molecular del Instituto de Biología Molecular y Celular de Rosario.El tema propuesto es la "Optimización de sistemas de expresión heteróloga de desaturasas de lípidos de tripanosomátidos".La idea sería clonar las acyl-CoA sintetasas de tripanosomátidos en levaduras con el fin de optimizar la generación de acyl-CoAs utilizados luego por desaturasas que se incluirían por co-transformación. La hipótesis es que este sistema aumentaría la eficiencia de la actividad de desaturasas heterólogas con el fin de caracterizar su actividad.En el laboratorio se ha caracterizado la vía de sintesis de ácidos grasos poliinsaturados en tripanosomátidos (Trypanosoma y Leishmania), con el fin de encontrar posibles blancos para el desarrollo de quimioterápicos, ya que estas víás son diferentes de las de mamíferos.El trabajo sería realizado bajo la dirección de la Dra Karina Trípodi.Como referencia del trabajo en el laboratorio pueden ver:Tripodi KE, Buttigliero LV, Altabe SG, Uttaro AD.
Functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan.
FEBS J. 2006 Jan;273(2):271-80.Uttaro AD.
Biosynthesis of polyunsaturated fatty acids in lower eukaryotes.
IUBMB Life. 2006 Oct;58(10):563-71. Review.Livore VI, Tripodi KE, Uttaro AD.
Elongation of polyunsaturated fatty acids in trypanosomatids.
FEBS J. 2007 Jan;274(1):264-74.Los interesados me pueden mandar un mail y los pongo en contacto con Karina.SaludosLic. Sebastián R. Najle Lab. de Parasitología Molecular - Depto. de Microbiología Instituto de Biología Celular y Molecular de Rosario (IBR-CONICET) Facultad de Ciencias Bioquímicas y Farmacéuticas Universidad Nacional de Rosario Suipacha 531 - S2002LRK, Rosario ARGENTINA

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